Synergistic effects of Bifidobacterium thermophilum RBL67 and selected prebiotics on inhibition of Salmonella colonization in the swine proximal colon PolyFermS model

Table 1 16S rRNA gene copy numbers of bacterial groups by qPCR in reactors during periods 2-4

	Bacterial group, log₁₀copies/mL effluent
Treatment	*Bifidobacterium thermophilum*	*Bifidobacterium*spp.	*Bacteroides-Prevotella*	*Enterobacteriaceae*	*Lactobacillus/Leuconostoc/Pediococcus*spp.	*Clostridium*Cluster IV	total 16S rRNA genes
PERIOD 2
Control	n.d.	6.4±0.2	10.3±0.1	9.6±0.1	7.6±0.1	10.0±0.1	10.5±0.1
N-15	n.d.	6.5±0.2	10.3±0.03	9.6±0.1	8.2±0.3^*	10.1±0.1	10.5±0.2
RBL67	7.6±0.1	8.3±0.2^*	10.2±0.1	10.0±0.1^*	7.6±0.2	10.0±0.1	10.6±0.2
FOS	n.d.	6.0±0.2^*	10.4±0.04	9.9±0.2^*	7.4±0.1	10.0±0.03	10.7±0.1
R-FOS	8.1±0.1	8.7±0.2^*	10.2±0.2	9.9±0.2	7.4±0.2^*	10.0±0.1	10.7±0.1
PERIOD 3
Control	n.d.	6.7±0.1	10.0±0.03	10.0±0.1	8.2±0.3	10.0±0.02	10.6±0.1
N-15	n.d.	6.9±0.2	10.1±0.2	9.8±0.1^*	8.2±0.2	10.1±0.2	10.6±0.2
RBL67	7.7±0.1	7.7±0.1^*	10.1±0.2	10.2±0.1^*	8.6±0.2	10.1±0.1	10.6±0.2
GOS	n.d.	6.6±0.04	9.9±0.2	9.7±0.2^*	8.1±0.2	10.0±0.5	10.5±0.1^*
R-GOS	7.7±0.1	8.0±0.1^*	10.0±0.2	9.9±0.2	8.3±0.3	10.1±0.3	10.5±0.2
PERIOD 4
Control	n.d.	6.4±0.1	10.0±0.2	10.0±0.2	8.0±0.2	9.5±0.02	10.6±0.3
N-15	n.d.	7.0±0.1^*	9.9±0.1	9.8±0.1	8.1±0.3	9.8±0.1	10.5±0.1
R-MOS	7.6±0.1	8.1±0.1^*	9.8±0.3	9.8±0.2	8.2±0.4	9.5±0.3	10.5±0.4
R-FOS	8.0±0.1	8.4±0.1^*	9.8±0.1	9.9±0.2	8.4±0.2^*	9.7±0.1	10.5±0.1
R-GOS	7.8±0.1	8.3±0.03^*	9.9±0.1	10.0±0.2	8.3±0.2	9.8±0.2	10.6±0.3

^*Bacterial populations significantly different (P?<?0.05) from CR within a period; Values are given as means±SD (log₁₀ copy numbers/mL reactor effluent) calculated from three consecutive days (days 24) during N-15 challenge periods.
N-15: Salmonella N-15 without pro- or prebiotic; RBL67: B. thermophilum RBL67 alone; FOS: FOS alone; R-FOS: B. thermophilum RBL67 + FOS; GOS: GOS alone; R-GOS: B. thermophilum RBL67 + GOS; R-MOS: B. thermophilum RBL67 + MOS.

ISSN: 1757-4749