Fig. 3

PCR amplification of mcr-1 gene in colistin resistant E. coli (Lane M, DNA size marker (100-1000Â bp); Lane 1: positive control (plasmid template), Lane 2/3/5/6: mcr-1 positive isolates (plasmid template) (309Â bp), Lane 4: negative control (no template) and Lane 7/8 mcr-1 negative isolates