Fig. 7

Increase in Treg cells expressing IL-10 and IL-22 after end-to-end and Roux-en-Y anastomoses. Intestinal mucosal cells were harvested 3 days post-operatively and stained extracellularly with TCRβ and CD4 and intracellularly with FoxP3, IL-10, and IL-22. Samples were then run on either a BD LSRII or Symphony. A Significant increase in normalized Treg (TCRβ+ CD4+) cell numbers and Treg cells that are B IL-22+ or C IL-10+. *p  ≤  0.05–no surgery, n  =  6, 7