Fig. 3

The ability for interaction of S. flexneri serotypes in the absence or presence of azithromycin and ciprofloxacin. The ability for interaction of S. flexneri serotypes was measured in the absence or presence of azithromycin (Azt, 32 µg/mL) (A) and ciprofloxacin (Cip, 4 µg/mL) (B) during 30 min. Data are expressed as mean of colony forming units (cfu)/cell ± std of HT-29 cell line (105 Cells/well) from two independent experiments. Confluent cells in duplicate wells were inoculated with fresh cultures of S. flexneri strains at MOI of 100 (107 cfu). The interaction rate for each assay was calculated based on number of grown colonies from cell lysates (CFU) on Muller Hinton Agar plates per each cell in tissue culture plates after treatment time