Fig. 5

NAD⁺ supplementation reversed the inhibition of F. nucleatum on the antioxidant capacity of L02 cells. a Immunoblotting analysis on the protein levels of SIRT1 in L02 cells treated with different concentrations of NAD.⁺ and stimulated with TNF-α (100 ng/mL), D-Gal (44 μg/mL) and F. nucleatum for 24 h. b ROS productions were detected by DHE staining. Representative images of the DHE staining in different groups. c ROS productions were evaluated by quantification of mean fluorescence intensity in DHE staining. d–f Levels of Malondialdehyde (MDA), Superoxide dismutase (SOD) and Glutathione peroxidase (GSH‐Px) in L02 cells. Data shown are means ± SD of three separate experiments. *P < 0.05; one-way ANOVA combined with Bonferroni's post hoc test; the error bars indicate the SDs