Fig. 1

Schematic representation of study design. STZ-induced T1DM (model) mice were given normal laboratory chow diet and normal water throughout the experiment. Adaptation to laboratory environment (day 0 to day 7), followed by intraperitoneal injection of STZ (50 mg/kg body weight) to overnight fasted mice for 5-consecutive days (day 8 to day 12). The mice were divided into six groups (n = 10 mice in each group): control group (normal healthy control), model group (STZ-induced T1DM), metformin group (200 mg/kg body weight), DDP-Low: low dose of CDDP (200 mg/kg body weight), DDP-Med: medium dose of CDDP (400 mg/kg body weight) and DDP-High: high dose of CDDP (600 mg/kg body weight) after the monitoring for 3-days (day 13 to day 15) for diabetes development. From day 16 to day 43 (week 1 till week 4), the mice were orally gavage with crude polysaccharide (CDDP) and metformin HCl. Weekly collection of feces, followed by record of fasting blood glucose levels, body weights, food consumption and water intake for 4-weeks of the study duration