Fig. 3

Interleukin (IL)-1β and IL-6 mRNA expression in CIECs after inoculation with C. jejuni. CIECs were inoculated with 106 CFU/ml of one of three different C. jejuni strains (11168, Lior6, or 0097). Cells were collected four and eight hours post inoculation, and IL-1β (a) and IL-6 (b) mRNA expression was investigated. The results are presented as the fold change in expression compared to that in noninoculated control cells. Relative quantification was performed by qRT‒PCR, and expression values were normalized to 28 S rRNA. Error bars indicate the standard error of the mean (SEM). Letters indicate significant differences between strains. (n = 5–7 replicates; p < 0.05; one-way analysis of variance with Tukey’s HSD All-Pairwise Comparisons Test). Asterisks indicate significant differences compared to the noninoculated controls at the investigated time points (p < 0.05; two-sample t test)