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Table 1 Number of sacrified chickens, trials and total replications

From: In vitro investigations on interference of selected probiotic candidates with Campylobacter jejuni adhesion and invasion of primary chicken derived cecal and Caco-2 cells

Experimental

Part

Experiment

Description

Used cells

Sacrificed

Chickens1

Number of

trials

Number of

replicates2

CIEC

Caco-2

1

1

Colonisation pattern after infection with different concentrations of C. jejuni 11168

+

+

9

2-3

6-9

1

2

Colonisation pattern after infection of CIEC with three different C. jejuni strains

+

 

8

3

9

1

3

IL-1β and IL-6 mRNA expression in CIEC after C. jejuni infection

+

 

3

1

5-7

2

4

Agar Well Diffusion Assay

  

0

1

3

3

5

Influence of incubation time point of probiotic candidates on colonisation of CIEC by C. jejuni 11168

+

 

5

2

6

3

6

Influence of incubation time point of probiotic candidates on colonisation of Caco2 cells by C. jejuni 11168

 

+

0

2

6

3

7

Influence of probiotic candidates on colonisation of CIEC by different C. jejuni strains

+

 

6

2

6

3

8

Influence of probiotic concentration on colonisation of CIEC by C. jejuni 11168

+

 

7

2

6

  1. 1: Number of total sacrificed SPF-chickens per experiment used to prepare CIEC. Isolated cells were pooled within a trial.
  2. 2: Number of total replicates per treatment group and time point investigated.