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Fig. 1 | Gut Pathogens

Fig. 1

From: The C-terminal proline-rich repeats of Enteropathogenic E. coli effector EspF are sufficient for the depletion of tight junction membrane proteins and interactions with early and recycling endosomes

Fig. 1

Expression of PRR domains of EspF depletes the total levels of TJ proteins. (A) Schematic representation of mutant EspF constructs. (B) Cell lysates obtained from wild type MDCK cells or stable cells lines expressing GFP vector (AcGFP), GFP-EspF (EspF), GFP-PRR-1-2-3 (containing all three PRRs), GFP-PRR-2-3 (containing PRR-2-3) and GFP-PRR-3 (containing only PRR-3) were analyzed by Western blotting. (C) Band intensities were measured by ImageJ software and fold change in the expression of each protein with respect to MDCK cells (normalized to 1) was plotted relative to GAPDH. Three cell lines were analyzed for each EspF construct and experiments were performed at least three times. A representative blot from one experiment is shown. Bars represent means ± s.e.m from three independent experiments; **p value < 0.005 and ***p value < 0.0005. No change was seen in the levels of the TJ adaptor ZO-1 or actin (not shown in panel C)

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Gut Pathogens

ISSN: 1757-4749