Fig. 2

Cryptosporidium parvum excystation in the human in vitro upper gastrointestinal tract. (A) Flow cytometry gating strategy on forward-angle light scatter/side-angle light scatter established during in vitro excystation of parasites, allowing detection of intact oocysts, empty oocyst shells and sporozoites. Created with BioRender.com (Agreement number: FF26ZFMVSM). (B) Parasite excystation during child or adult digestion in the TIM-1 system. Cytograms obtained for one representative experiment for the child or the adult digestion at 45Â min in the stomach and at 60Â min in the duodenum show the timing when excystation occurs in the in vitro gastrointestinal tract