Exploring the impact of digestive physicochemical parameters of adults and infants on the pathophysiology of Cryptosporidium parvum using the dynamic TIM-1 gastrointestinal model

Table 1 Parameters of gastrointestinal digestion in the TIM-1 system under adult or infant conditions

Parameter	Adult	Infant
pH	Gastric compartment (min/pH)	Gastric compartment (min/pH)
	t = 10 → 3.2	t = 10 → 5.7
	t = 20 → 2.4	t = 20 → 5.3
	t = 40 → 1.8	t = 40 → 4.5
	t = 60 → 1.6	t = 60 → 3.2
	t = 90 → 1.5	t = 90 → 2.0
	Duodenal compartment: 6.4	Duodenal compartment: 6.4
	Jejunal compartment: 6.9	Jejunal compartment: 6.9
	Ileal compartment: 7.2	Ileal compartment: 7.2
Digestive secretions	Gastric compartment
	130 IU/min of pepsin 10 IU/min of lipase 0 to 0.25 mL/min of HCl 0.5 M (according to pH)	130 IU/min of pepsin 10 IU/min of lipase 0 to 0.25 mL/min of HCl 0.3 M (according to pH)
	Duodenal compartment
	1 mL/min Bile salts^a 27.9 mM during the first 30 min and then 9.3 mM 0.5 mL/min Pancreatin 4USP juice 44.6 g/L Trypsin^b: 3 mg 0.5 mL/min of intestinal electrolytes solution^c combined to NaHCO₃ 0.5 mol/L if necessary (according to pH)	1 mL/min Bile salts^a 14 mM during the first 30 min, then 4.67 mM 0.5 mL/min Pancreatin 4USP juice 22.3 g/L Trypsin^b: 2.4 mg 0.5 mL/min of intestinal electrolytes solution combined to NaHCO₃ 0.5 mol/L if necessary (according to pH)
	Jejunal compartment
	0 to 0.25 mL/min of NaHCO₃ 0.5 mol/L (according to pH)	0 to 0.25 mL/min of NaHCO₃ 0.5 mol/L (according to pH)
	Ileal compartment
	0 to 0.25 mL/min of NaHCO₃ 0.5 mol/L (according to pH)	0 to 0.25 mL/min of NaHCO₃ 0.5 mol/L (according to pH)

^aBile is composed of a mixture of bile extract porcine and bile salts (sodium deoxycholate and sodium cholate hydrate)
^bintroduced into the duodenum compartment at t = 0
^cElectrolytes solution is composed of 7 g/l NaCl, 0.6 g/L KCl, and 0.3 g/L CaCl2 dihydrate

ISSN: 1757-4749