Efficacy of colistin-based combinations against pandrug-resistant whole-genome-sequenced Klebsiella pneumoniae isolated from hospitalized patients in Egypt: an in vitro/vivo comparative study

Table 2 Colistin MIC and the genetic alterations associated with colistin resistance in pandrug-resistant K. pneumoniae isolates

Isolate code	COL^a MIC (µg/mL)	PhoQ	PmrA	PmrB	PmrC	PmrD	CrrB	mgrB / MgrB	ArnB	ArnA	ArnD	ArnT	ArnF	Others^c
KP11	128	WT^b	WT	A223T G233R	F27C S257L R319Q	WT	Absent	ISKpn14 at position 35	A112D I126V G47D	WT	S164P	WT	WT	WT
KP8	8	E397G	N131D G144D D149E N219H	N82S M152V A205T G233R	F27C L39V R319Q	T60M	Absent	WT/WT	A112D D217N D285E	WT	WT	I117V	F48V	WT
KP3	16	WT	N131D G144D D149E N219H	N82S M152V A205T G233R	F27C L39V R319Q	T60M	Absent	G109A/G37S	A112D D217N D285E	WT	WT	L54H I117V	F48V	WT
KP20	512	WT	WT	WT	V50L A135P R319Q	WT	V194_S195insSS^d S196G S197G G198A A200Y	WT / WT	WT	S18A T185A	WT	L114M I117V H156Q	WT	WT
KP22	16	WT	WT	WT	V50L A135P R319Q	WT	WT	C88T/Q30*	WT	S18A T185A	WT	L114M I117V H156Q	WT	WT

^a COL colistin, ^b WT wild-type, ^c Others refer to PhoP, Kdgr, YobH, YobF, YebO, CspC, RamA, ArnC, and ArnE. ^dV194_S195insSS indicates the insertion of two serine amino acids between valine at position 194 and serine at position 195. *Represents a stop codon. The bold format indicates deleterious amino acid substitution predicted by PROVEAN and the genetic alterations associated with colistin resistance

ISSN: 1757-4749