Fig. 3

Maximum Likelihood (ML) unrooted tree with the highest log likelihood (−804.94), based on partial sequences of the tpi gene of Giardia duodenalis, generated using the Kimura 2-parameter model (K2P) [53]. G. duodenalis assemblages of the current study were colored with three distinct colors: Orange for genotype A, blue for genotype B and pink for genotype E. The dataset comprised 38 sequences: 28 sequences of tpi gene obtained in this study (A25-D52, Bold) were compared with ten reference sequences of known sub-assemblage from GenBank. Next to the branches, the proportion of trees (1000 replicates) in which the related taxa clustered together is displayed. Entire bootstrap value > 50% is displayed. The branch lengths of the scaled-up tree are expressed in terms of the number of substitutions made at each site. There were 283 positions in the final dataset. POCT: Patients on corticosteroid therapy; CONT: Controls