Fig. 4

Maximum Likelihood (ML) unrooted tree with the highest log likelihood (−648.64), based on partial sequences of the bg gene of Giardia duodenalis, generated using the Tamura-Nei substitution model (TrN) [54]. G. duodenalis assemblages of the current study were coloured with three distinct colours: Orange for genotype A, blue for genotype B and pink for genotype E. The dataset comprised 29 sequences: 19 sequences of bg gene obtained in this study (B01-D52) were compared with ten reference sequences of known sub-assemblage from GenBank. Next to the branches, the proportion of trees (1000 replicates) in which the related taxa clustered together is displayed. Entire bootstrap value > 50% is displayed. The branch lengths of the scaled-up tree are expressed in terms of the number of substitutions made at each site. There were 327 positions in the final dataset. POCT: Patients on corticosteroid therapy; CONT: Controls.