Fig. 4

Intestinal cytokines were analyzed using CBA and ELISA in C57BL/6 mice, whether infected or not with T. gondii, and treated or not with SGE (30 µg) during chronic or acute infection phases. Fragments of the small intestine were collected and homogenized in RIPA buffer and adjusted to a concentration of 200 mg/mL. Data are expressed as the mean and standard deviation of cytokine amounts. A TNF, B IFN-γ, C MIF, D IL-4, E IL-10, F IL-2, G TGF, H IL-17, and (I) IL-6. # Represents significant differences between treated and untreated groups in the same experimental condition (treated and untreated mice). *Represents differences between different experimental conditions (uninfected, acute infection, and chronic infection). Tests were performed using 2-way ANOVA followed by Tukey’s multiple comparison post-test, and differences were considered significant when * = 0.05, ** = 0.01, *** = 0.001. # = 0.05, ## = 0.01, ### = 0.001 (n = 10 mice per group). IFN-γ, TNF, IL-17, IL-6, IL-4, IL-2, and IL-10 were measured by CBA, whereas IL-12, MIF, IL-5, TFG-β, IL-22, and IL-23 were measured by ELISA