Fig. 4

Agarose gel electrophoresis of nested PCR amplification products targeting Cryptosporidium spp. GP160 gene. Wells: 1 molecular weight marker. 2: Positive control (reference DNA from C. parvum). 3: Negative control (reaction mixture without DNA). 4: Negative extraction control. 23, 31, 33, and 37: positive samples. 34 and 36: Faint bands indicate weak positive results. 5–11, 13–22 and 24–29: Negative samples