Fig. 5

Impact of 1,2PD on the membrane fluidity and composition of C. perfringens. The impact of 1,2PD on membrane fluidity was determined using Laurdan while composition of membrane lipids after incubation in YC, YC-G50 and YC-PD50 for 24 h at 37 °C was analysed using LC-MS/MS. (A) Effect of 1,2PD on general polarisation (GP) of the membrane. Shown are the differences in GP levels in treatment versus the control (PBS only). Experiments were conducted in biological triplicates. (B) Peak intensities of major membrane lipid components. Shown are mean values of biological duplicates. (C) Log2 fold change of lipids in YC-PD50 (50 mM) compared to YC-G50 (50 mM) obtained from DESeq analysis in C. perfringens FMT 1006 cultured in YC-G50 (50 mM) or YC-PD50 (50 mM). Different letters denote significant difference between conditions (p < 0.05, Dunn’s Test adjusted by Holm). Significance by Wald test (p < 0.05) is indicated with (*). PE: Phenylethanol. PD: 1,2-propanediol. Diacylglycerols (DAG), monoacylglycerols (MAG), glycerophosphoethanolamines (GPE) and triacyclglycerols (TAG)