Table 3 Relative expression of selected pdu genes. RNA isolated from C. perfringens FMT 1006 after 3–4 h cultivation in YC, YC-G50 and YC-PD50. cDNA was prepared from RNA and gene expression was determined by qPCR using cDNA as template. For standardisation, expression of target genes was calculated relative to the geometric mean of housekeeping genes ftsZ, gyrA and recA, and to expression in YC-G50. RNA was collected from two independent experiments, and each sample was analysed in technical duplicates

From: Insights into the utilisation of 1,2-propanediol and interactions with the cell envelope of Clostridium perfringens