Fig. 3

Liver microbial diversity and composition of HBs-HepR mice compared with control B6-HepR mice. Liver samples were harvested from HBs-HepR (n = 3), B6-HepR (n = 6) and Fah−/− control mice (n = 4). 2bRAD-M sequencing was used to analyze the microbiota. A Venn diagram of ASVs. The number of ASVs in each group and the number of shared ASVs among the three groups are shown. B Alpha diversity was evaluated using the Wilcoxon rank sum test. C Beta diversity was evaluated using PCoA with binary Jaccard. A dot represents one sample. D Compositions of the microbiota community at the phylum level in each group. (E) Circos diagram illustrating the relationships between samples and species